The current research aims to develop and validate a scale to measure tolerance in spouses’ relations based on the Islamic approach. The items of the scale were prepared using religious texts (Quranic verses, Sayings, and Religious Interpreters' Comments) and the validity of the scale was verified by a number of religious experts and family psychologists. Development and evaluation of psychometric properties of the scale was performed in five studies. In the first study, the reliability of the initial scale was calculated using Cronbach's alpha as .93 in a sample of 80 married men and women. In the second study, using a sample of 540 married men and women, 4 factors (acceptance and empathy toward spouse, emotional control and patience, facilitating the process of spouse's growth and actuation toward spouse, and beneficence and forgiveness toward spouse) were identified using exploratory factor analysis (EFA). Also in the second study, the reliability coefficients of each factor was calculated using Cronbach's alpha coefficient and all of indices were indicative of suitable internal consistency of the scale. In the third study, using a sample of 100 married women, the convergent validity of the scale was confirmed, and in the fourth study, using a 30-member sample, the reliability of the scale was confirmed using test re-test method. In the fifth study, using a sample of 369 married men and women, the four-factor scale of the scale was confirmed using confirmatory factor analysis (CFA). According to the results, the Tolerance in Spouses’ Relations Scale (TSRS) can be used as a valid tool for measuring tolerance in spouses’ relations.